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. 2019 Jun 26;8:e45418. doi: 10.7554/eLife.45418

Figure 2. Cleft palate defects in Rbfox2 mutant embryos.

Gross morphology and Alcian blue staining of control (A, C and E) and Rbfox2Pax3-CKO (G, I and K) palates at E12.5 (n = 7 controls, n = 7 Rbfox2Pax3-CKO), E15.5 (n = 6 controls, n = 6 Rbfox2Pax3-CKO) and E18.5 (n = 7 controls, n = 7 Rbfox2Pax3-CKO). H and E stained transverse sections of control (B), (D), and (F) and Rbfox2Pax3-CKO (H), (J), and L) embryos at the level of the anterior and posterior palatal shelves. Gross morphology and Alcian blue staining of control (M and O) and Rbfox2Wnt1-CKO (Q and S) palates at E15.5 (n = 6 controls, n = 6 Rbfox2Wnt1-CKO) and E18.5 (n = 7 controls, n = 6 Rbfox2Wnt1-CKO). H and E stained sections of control (N and P) and Rbfox2Wnt1-CKO (R and T) E15.5 and E17.5 embryos at the level of the anterior and posterior palatal shelves. Asterisks (*) represent cleft palate in Rbfox2 mutant embryos (I–L and Q–T). P, palate; PS, palatal shelves; NS, Nasal septum; T, Tongue. Scale bars are 200 μm respectively.

Figure 2.

Figure 2—figure supplement 1. Rbfox2 deletion leads to neonatal lethality.

Figure 2—figure supplement 1.

Genotyping results from embryos and pups generated by a cross between Pax3Cre/+; Rbfox2flox/+ and Rbfox2flox/flox mice (A). Gross morphology and histology of control (B–F) and mutant (G–K) embryos and pups at different embryonic and neonatal time points. Pax3Cre/+;Rbfox2flox/flox (Rbfox2Pax3-CKO) embryos show subcutaneous edema (white arrows) compared to controls (n = 40 controls, n = 40 Rbfox2Pax3-CKO). H and E staining of sections detects cleft palate (red arrow) and thin dermal layer in the skin of Rbfox2Pax3-CKO embryos (black arrow) than in littermate controls due to subcutaneous edema (n = 5 controls, n = 5 Rbfox2Pax3-CKO) (F and K). Scale bars are 1 mm respectively. Hematoxylin and eosin stained sagittal sections of control (L, N) and Rbfox2Pax3CKO (M, O) pups at P1 (n = 4 each genotype). Scale bars are 200 μm respectively. Genotyping results from embryos and pups generated by a cross between Wnt1Cre/+;Rbfox2flox/+ and Rbfox2flox/flox mice (P). Gross morphology of control (Q) and Rbfox2Wnt1-CKO (R) pups at P1 (n = 4 each genotype). The mutant lungs are not inflated compared to controls. A, atrium; V, ventricle; Lu, lung.