Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | Rbfox2flox/flox | (Gehman et al., 2012) | IMSR Cat# JAX:014090, RRID:IMSR_JAX:014090 | |
| Genetic reagent (M. musculus) | Pax3Cre/+ | (Engleka et al., 2005) | IMSR Cat# JAX:005549, RRID:IMSR_JAX:005549 | |
| Genetic reagent (M. musculus) | Wnt1Cre2 | (Lewis et al., 2013) | IMSR Cat# JAX:022137, RRID:IMSR_JAX:022137 | |
| Genetic reagent (M. musculus) | R26mTmG/+ | (Muzumdar et al., 2007) | IMSR Cat# JAX:007676, RRID:IMSR_JAX:007676 | |
| Antibody | anti-Tak1 (mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-166562, RRID:AB_2140220 | WB (1:300) |
| Antibody | anti-Fibronectin (mouse monoclonal) |
Santa Cruz Biotechnology | Cat# sc-8422, RRID:AB_627598 | WB (1:300) |
| Antibody | anti-β-actin (mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-47778, RRID:AB_626632 | WB (1:1000) |
| Antibody | anti-pTak1 (rabbit polyclonal) | Cell Signaling Technology | Cat# 9339, RRID:AB_2140096 | WB (1:500) |
| Antibody | anti-Smad2-C (rabbit monoclonal) | Cell Signaling Technology | Cat# 5339, RRID:AB_10626777 | WB (1:500) |
| Antibody | anti-pSmad2-C (rabbit monoclonal) | Cell Signaling Technology | Cat# 3108, RRID:AB_490941 | WB (1:500) |
| Antibody | anti-p38 Mapk (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9212, RRID:AB_330713 | WB (1:500) |
| Antibody | anti-p-p38 Mapk (rabbit monoclonal) |
Cell Signaling Technology |
Cat# 4631, RRID:AB_331765 | WB (1:500) |
| Antibody | anti-Rbfox2 (mouse monoclonal) |
Abcam | Cat# ab57154, RRID:AB_2285090 | WB (1:500), IF (1:25) |
| Antibody | anti-Ki67 (rabbit monoclonal) |
Abcam | Cat# ab16667, RRID:AB_302459 | IHC (1:100) |
| Antibody | anti-αSMA (mouse monoclonal) |
Sigma-Aldrich | Cat# A2547, RRID:AB_476701 | IHC (1:100) |
| Antibody | anti-Neurofilament (mouse monoclonal) |
DSHB | Cat# 2H3, RRID:AB_531793 | Wholemount staining (1:50) |
| Recombinant DNA reagent | pCMV5B-HA-Smad2 plasmid | Addgene | RRID:Addgene_11734 | |
| Recombinant DNA reagent | pCMV5B-Flag-Smad3 plasmid | Addgene | RRID:Addgene_11742 | |
| Recombinant DNA reagent | pCMV5B-Smad4 plasmid | Addgene | RRID:Addgene_11743 | |
| Recombinant DNA reagent | pcDNA3-TAK1/F plasmid | Addgene | RRID:Addgene_44161 |