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. 2019 Jun 27;8:e46207. doi: 10.7554/eLife.46207

Figure 8. Co-assembly of ESCRT-III proteins into membrane-deforming polymers drive budding reactions.

(A) Figure on the left depicts a model of ESCRT-III spirals budding membranes by utilizing different curvatures. Figure on the right depicts how laterally associating polymers can reposition themselves utilizing electrostatics at different curvatures. In the figures, H represent a position of high curvature and L represents a position of low curvature. (B) Model depicting different steps of the simultaneous recruitment of Snf7/Vps24/Vps2, which form spirals that drive membrane budding reactions.

Figure 8.

Figure 8—figure supplement 1. Comparison of inside-out and outside-in tubulation properties of ESCRT-III proteins.

Figure 8—figure supplement 1.

(A) Hypothetical model of how the Snf7/Vps24/Vps2 polymers engage membranes (right), compared to the structure-based model of CHMP1B-IST1 assembly (left). (B) Figure illustrates a hypothetical model of how the helices of Snf7 and its partners may be arranged on the membrane. Note that only helices 1–3 are shown in this model. Arrows point to the different positions of helices along the polymer.