(A) Ratio of 25(OH)D3 to cholecalciferol is increased in lean mice relative to obese mice. The ratio of 25(OH)D to cholecalciferols is increased in lean mice relative to obese mice (n ≥ 3 for each group, all mice 21 to 24 weeks of age, ***
p < 0.001 by t test). (B) The ratio of 25(OH) D3 to cholecalciferol correlates with liver Cyp2r1 mRNA abundance. Ratio = –0.1828 + 0.7166* CYP2R1, R2 = 0.858 (n ≥ 3 for each group, all mice 21 to 24 weeks of age, ***
p < 0.001 by t test). Red = obese mice on high fat diet; Blue = lean mice on CHOW diet.