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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Chembiochem. 2019 May 3;20(13):1659–1663. doi: 10.1002/cbic.201800817

Figure 2.

Figure 2.

5HTP-4CDZ azo dye can quench sfGFP fluorescence. A) The scheme for expressing sfGFP-151-5HTP and its subsequent labeling with 4CDZ. B) ESI-MS analysis of sfGFP-151-5HTP before and after treatment with 4CDZ confirm selective labeling. C) Normalized fluorescence spectra of sfGFP-151-5HTP before and after 4CDZ labeling show robust quenching of fluorescence. Reduction of the azo-linkage by dithionite results in a significant recovery of the sfGFP fluorescence. Images of these samples under trans-illumination are also shown.