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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Arthritis Rheumatol. 2019 Jun 12;71(8):1350–1359. doi: 10.1002/art.40890

Figure 3. CYR61 promotes angiogenesis in diffuse cutaneous SSc ECs through αvβ3, eNOS, AMPK, and AKT.

Figure 3.

(A) CYR61 mRNA levels were significantly lower in dSSc ECs compared to normal ECs. (B) Overexpression of CYR61 in dSSc ECs led to significant increase of CYR61, VEGF, and NOS3. (C-D) Significant elevation of CYR61 and VEGF was observed in culture media from CYR61-overexpressing dSSc ECs. (E-F) ITGAV and ITGB3 mRNA expression levels were elevated in dSSc ECs compared to normal ECs. (G) Significant elevation of phosphorylated eNOS, AKT, and AMPK was observed in CYR61 transfected dSSc ECs. (H) Metabolites of NO, nitrates and nitrites, were increased in culture media collected from CYR61 transfected dSSc ECs. n=number of patients. Results are expressed as mean +/− SD and p<0.05 was considered significant.