Table 2.
Reagents used for OMIP‐056
| Detector | Fluorochrome | Specificity | Clone | Purpose |
|---|---|---|---|---|
| B515 | FITC | Perforin | B‐D48 | Function |
| B660 | BB660 | CD14 | MϕP9 | Monocytes |
| B710 | BB700 | IL2 | MQ1‐17H12 | Function |
| G575 | PE | IL22 | 22URTI | Function |
| G610 | PE‐Dazzle 594 | KLRG1 (MAFA) | SA231A2 | Differentiation |
| G660 | PE‐Cy5 | CXCR3 | 1C6/CXCR3 | T helper class |
| G710 | PE‐Cy5.5 | CD56 | CMSSB | NK, NKT |
| G780 | PE‐Cy7 | CD154 | 24–31 | Function |
| R660 | APC | IL4 | MP4‐25D2 | Function |
| IL13 | JES10‐5A2 | |||
| R710 | Alx700 | Granzyme A | CB9 | Function |
| R780 | APC‐Cy7 | TCRvα7.2 | 3C10 | MAIT |
| U395 | BUV395 | CD3 | UCHT1 | T cell lineage |
| U450 | UViD | Viability | NA | Viability |
| U500 | BUV496 | CD45RA | HI100 | Differentiation |
| U570 | BUV563 | CD8 | RPA‐T8 | T cell lineage |
| U660 | BUV661 | HLA‐DR | G46‐6 | Activation |
| U730 | BUV737 | IL17a | N49‐653 | Function |
| U780 | BUV805 | CD4 | RPA‐T4 | T cell lineage |
| V450 | V450 | IFNy | B27 | Function |
| V510 | BV510 | TCR γδ | 11F2 | γδ T cell lineage |
| V570 | BV570 | CD16 | 3G8 | NK, NKT |
| V610 | BV605 | CCR6 (CD196) | 11‐A9 | T helper class |
| V655 | BV650 | CD161 | DX12 | MAIT |
| V710 | BV711 | CD26 | M‐A261 | MAIT |
| V750 | BV750 | TNFα | MAb11 | Function |
| V780 | BV785 | CCR7 (CD197) | G043H7 | Differentiation |
APC, allophycocyanin; Alx, Alexa; BB, brilliant blue, BUV, brilliant ultraviolet; BV, brilliant violet; Cy, cyanine; FITC, fluorescein isothiocyanate; PE, R‐phycoerythrin; UViD, LIVE/DEAD fixable ultraviolet dead cell stain.