Figure 1.

Tyrosine nitration is increased in NF2 schwannoma cells. A, representative IR Western blots showing nitrotyrosine staining of vestibular schwannoma samples from three NF2 patients (VS 1 to 3), and human (I) and mouse (MSC) WT- and MD-Schwann cells. α-Tubulin and β-actin were used as loading controls. B, quantitation of nitrotyrosine levels in HSC (n = 4). C, loss of merlin expression in both human and mouse MD-Schwann confirmed by IR Western blotting. D–F, representative Western blots showing the levels of: D, nNOS (n = 3–4); E, inducible and endothelial NOS (iNOS and eNOS, respectively, n = 4–5); and F, MnSOD (n = 7–8) in human and mouse WT- and MD-Schwann cells. Homogenate from human WT-Schwann cells treated with 1 μm lipopolysaccharide (LPS) for 24 h was used as positive control for iNOS expression, and brain homogenate as control for nNOS and eNOS expression. Columns represent the mean ± S.D. of the respective Western blotting band quantitation normalized against α-tubulin or β-actin and expressed relative to WT-Schwann cells. *, p < 0.05 versus WT by either Student's t test or Mann-Whitney test.