Table 1.
SAXS data collection and scattering-derived parameters
| ACMSD | ACMSD H110A | |
|---|---|---|
| Data collection | ||
| Beamline | SSRL BL4-2 | SSRL BL4-2 |
| Type of experiment | SEC-SAXS | SEC-SAXS |
| Beam defining slits size (mm) | 0.3 × 0.3 | 0.3 × 0.3 |
| Sample–detector distance (m) | 1.7 | 2.5 |
| Beam energy (keV) | 11.4 | 10.1 |
| Beam current (mA) | 500 | 500 |
| Exposure time per frame (s) | 1 | 1 |
| Frames per data set | 500 | 500 |
| Temperature (K) | 298 | 298 |
| Sample cell size (mm diameter) | 1.5 | 1.5 |
| Detector | Pilatus 3 X 1M | Pilatus 3 X 1M |
| SEC column | Superdex 200 Increase PC3.2/300 | Superdex 200 Increase PC3.2/300 |
| Sample volume (Concentration) | 35 μl (4 mg/ml) | 50 μl (4 mg/ml) |
| Flow rate (ml/min) | 0.05 | 0.05 |
| Guinier analysis | ||
| q × Rg limita | 1.3 | 1.3 |
| I(0) (cm−1) | 0.0612 ± 0.0011 | 0.0439 ± 0.0012 |
| Rg (Å) | 47.0 ± 1.06 | 52.2 ± 1.95 |
| P(r) and Porod volume estimation | ||
| Software | Primus/GNOM | Primus/GNOM |
| qmax (Å−1) | 0.30 | 0.15 |
| I(0) (cm−1), real space | 0.0610 ± 0.0010 | 0.0454 ± 0.0006 |
| Rg, real space (Å) | 48.28 ± 0.98 | 55.99 ± 0.84 |
| Dmax (Å) | 175 | 190 |
| Porod volume (Å3) | 195,000 | 238,000 |
| Expected mass (kDa) | 158.8 | 158.8 |
| Rigid-body modeling | ||
| Software | CORAL | CORAL |
| qmax (Å−1) | 0.3 | 0.2 |
| Number of runs | 20 | 20 |
| Ab initio modeling | ||
| Software (ab initio modeling) | DAMMIF | GASBOR |
| Software (averaging) | DAMAVER | DAMAVER |
| qmax (Å−1) | 0.18 | 0.15 |
| Number of runs | 20 | 20 |
| Normalized spatial discrepancy (NSD) | 0.763 ± 0.038 | 2.272 ± 0.242 |
aq = 4πsin(θ)/λ, where 2θ is the scattering angle.