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MST assays for quantification of binding between porcine STINGCBD protein and four CDNs. The normalized change in the fluorescence of the labeled porcine STINGCBD protein was plotted against the CDN concentration. The experiments were performed in triplicate. Mean values ± S.D. (n = 3) are shown. A, 2′,3′-cGAMP. B, c-di-GMP. C, c-di-AMP. D, 3′,3′-cGAMP.
