Figure 4.
Initiation of reverse transcription is not augmented by the presence of RHA. Reactions contained tRNALys3 annealed to the PBS segment (333 nm) and ddATP (333 μm) instead of dATP, which terminates the RT reaction at the +6 nt position, and aliquots were collected and analyzed at the indicated time points. A, comparison of the +6 tRNALys3 primer extension products, synthesized in the absence and presence of RHA (1 μm), and separated by 8% urea–PAGE. Representative gels are shown. B, the amount of the +6 tRNALys3 product was quantified and normalized to total tRNALys3 to calculate the primer extension percentage. The average results of three independent experiments are shown with standard deviation (±).