Figure 7.

RHA enhances the processivity of RT. A, single turnover processivity assay sof RT using dsDNA trap in the absence (lane 1–9) and presence (lane 1′–9′) of RHA. RT (400 nm) was incubated with P/T (200 nm) or NC (3.2 μm) in the absence and presence of RHA (400 nm). The reactions were initiated with dNTPs (100 μm), MgCl₂ (5 mm), and dsDNA trap (0.3 μg). The reactions were carried out for 0, 20, 40, 60, 90, 120, 180, 240, and 300 s and loaded to lanes 1–9 and 1′-9′. Primer extension products were resolved in 20% urea–PAGE. B, the +1 products were quantified and fit into the single exponential decay equation. C, the +5 products were quantified and fit into the single exponential decay equation. D, the +6 products were quantified. The no RHA data were fit into the double exponential decay equation, and the RHA data were fit into the single exponential decay equation.