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. 2019 Jul 29;9:179. doi: 10.1038/s41398-019-0517-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Cal-520®-stained neurons of healthy control, schizophrenia, and autism groups were imaged for 10 min and signals obtained were used to calculate peak frequency (a), peak amplitude (b), peak area (c), and full width half maximum (d). Fluorescence intensity ΔF/F, frequency, and length of events were analyzed using Fiji and Origin software; Kruskal–Wallis Test and Dunn’s post hoc test, peak frequency H(2) = 22.56, ΔF/F0 H(2) = 19.44, peak area H(2) = 93.17, and FWHM H(2) = 45.2; *p < 0.01; ***p < 0.0001; for each group, error bars are s.e.m.