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(A) miR21 gene down-regulation of anti-miR21 nanopompons in vitro. Asterisks (*) denote statistically significant differences (data represented as mean±SD, **P < 0.001, n = 3). (B) QRT-PCR and (C) Western blot result of the down-regulation at both mRNA and protein level of miR21 after treatment, bringing about up-regulation of two target genes PTEN and PDCD4 in vitro. (D) Cell viability test of DHA-nanopompon, nanopompon, DHA-PEG-pOEI and PEG-pOEI on 293 cells by MTT assay.
