Table 3.
Immune profile of the SCID patient from the time of diagnosis up to the age of 7 years*.
| Result in time points (age in months) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 16 | 29 |
Normal range (0–3 yrs) |
44 | 60† |
Normal range (3–6 yrs) |
77‡ | 87 | Normal range (6–12 yrs) | |
| ALC/mm3 | 3,753 | 6,647 | 3,600–890 | 3,400 | 3,355 | 2,300–5,400 | 9,255 | 6,360 | 1,900–3,700 |
| LYMPHOCYTE SUBPOPULATION [ALC/mm3] | |||||||||
| CD19+/B lymphocytes | 150 | 598 | 720–2,600 | 884 | 101 | 390–1,400 | 185 | 191 | 270–860 |
| CD3+T lymphocytes | 1,989 | 2,061 | 2,100–6,200 | 1,054 | 1,845 | 1,400–3,700 | 6,664 | 4,833 | 1,200–2,600 |
| CD3+/CD4+ Th lymphocytes | 826 | 465 | 1,300–3,400 | 816 | 336 | 700–2,200 | 1,111 | 700 | 650–1,500 |
| CD3+/CD8+ Tc lymphocytes | 826 | 997 | 620–2,000 | 204 | 1,007 | 490–1,300 | 3,610 | 2,798 | 370–1,100 |
| CD3–/CD16+56+ NK Cells | 1,576 | 3,789 | 180–920 | 1,360 | 1,275 | 130–720 | 2,314 | 1,272 | 100–480 |
Surface markers for flow cytometry contained FITC-labeled CD3, PE-labeled CD16, and CD56, PerCP. CyTM5.5-labeled CD45, PE-CyTM7-labeled CD4, APC-labeled CD19, and APC-Cy7–labeled CD8. The samples were collected twice and experiments were repeated to rule out the inconsistency in the lymphocyte pattern. To maximize instrument performance and minimize inter-assay variability, CST (Cytometer Set-up and Tracking) beads were run daily on BD Facs-Aria machine. Yrs, years; ALC, absolute lymphocyte count.
Lymphocyte count of the last sample collected when the child was shedding VDPV3.
Lymphocyte count of the first sample collected after the child stopped VDPV3 shedding.