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. 2019 Jul 23;10:1620. doi: 10.3389/fimmu.2019.01620

Figure 1.

Figure 1

DIM suppresses the viability of RA-FLSs and arrests the cell cycle in G1 phase. (A) Molecular structure of (a) indole-3-carbinol (I3C) and (b) 3,3′-diindolylmethane (DIM). (B) The cell viability of RA-FLSs treated with DIM at various concentrations (0,20,40,60,80 μM) for 24 h and 48 h. (C) The cell cycle of RA-FLSs treated with DIM at various concentrations (0,30,60 μM) for 24 h. (D) Expression levels of CDK4, Cyclin D and p21 in RA-FLSs after treated with DIM (0,30,60 μM) for 24 h measured by Western blot(left panel). Representative images of immune blot and densitometric quantification of CDK4, Cyclin D and p21 expression(right panel). Data are represented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. 0 μM (Ctrl).