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. 2019 Jul 23;10:1620. doi: 10.3389/fimmu.2019.01620

Figure 2.

Figure 2

DIM Suppresses the migration and invasion of RA-FLSs. (A) The effect of DIM (0, 25, 50 μM) on migration was detected with transwell Boyden chamber after 6 and 12 h. The images are representative of migration or invasion through the membrane after staining. Original magnification 200 × (left panel). Cell numbers/field are presented as the mean ± SD of six independent fields (right panel). (B) The effect of DIM (0, 25, 50 μM) on invasion was detected with transwell Boyden chamber coated with a Matrigel basement membrane matrix after 12 and 24 h. The images are representative of migration or invasion through the membrane after staining. Original magnification 200 × (left panel). Cell numbers/field are presented as the mean ± SD of six independent fields (right panel). (C) The effect of DIM (0, 20, 40, 80 μM) on wound healing was detected with cell scratching assay. After 48 h the wound area was photographed using microscope. Original magnification 100 × (left panel). The extent of wound closure was presented as the percentage by which the original scratch width had decreased at each measured time point (right panel). The values are the mean ± SEM from at least 3 independent experiments. *P < 0.05, **P < 0.01 vs. 0 μM (Ctrl).