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. 2019 Jul 23;13:748. doi: 10.3389/fnins.2019.00748

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Copyright © 2019 Carrera-Cañas, Garzón and de Andrés.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Carbachol microinjection locations. (Upper) Photomicrograph of a brainstem coronal section stained with Nissl method from an animal with the two doses of carbachol and control-saline microinjections in the rostrodorsal pontine tegmentum at the level of the PLCα. The arrow indicates the site of the microinjections. (Lower) Coronal planes –2 and –3 of the Reinoso-Suarez (1961) atlas showing the location of the microinjection sites in the seven animals in which the PLCα nucleus was reached (stars). α, locus coeruleus alpha; BC, brachium conjunctivum; BP, brachium pontis; Cnf, cuneiform nucleus; IC, inferior colliculus; LC, locus coeruleus proper; LdT, laterodorsal tegmental nucleus; LL, lateral lemniscus; ML, medial lemniscus; M5, mesencephalic trigeminal tract; P, pyramidal tract; PLCα, perilocus coeruleus alpha; PaG, periaqueductal gray; vRPO, ventral part of the oral pontine reticular nucleus; SLC, locus subcoeruleus; SO, superior olivary nucleus; Tp, tegmental pontine nucleus; Tr, trapezoid body; TV, ventral tegmental nucleus.