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. 2019 Jul 23;10:803. doi: 10.3389/fphar.2019.00803

Figure 2.

Figure 2

MAPK pathway inhibition by PD98059 prevented the induction of ROS levels and profibrotic markers CTGF, TGF-β, and PAI-I. (A). For ROS quantification, M-1 cells were treated with PD98059 at 30 and 50 µM during 15 min, incubated with DCFH-DA probe for 30 min, and treated with hrPR for 15 min. Results are expressed as DCF probe fluorescence intensity versus total protein (mean ± SEM) (B). Protein levels of CTGF, TGF-β, and PAI-I represented by Western blot analysis (left) and quantitation (right) in M-1 cells incubated during 6 h with hrPR, PD98059, or hrPR plus PD98059, *p < 0.05 versus control, # p < 0,05 versus hrPR group, ‡ p < 0,05 versus positive control, n = 5.