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. 2019 Jul 23;10:803. doi: 10.3389/fphar.2019.00803

Figure 3.

Figure 3

NOX-4 inhibition by pharmacological agent GKT 137831 prevented the induction of ROS and profibrotic markers CTGF, TGF-β, and PAI-I. (A). For ROS quantification, M-1 cells were treated with GKT at 10, 20, and 30 µM during 15 min, incubated with DCFH-DA probe for 30 min, and treated with human recombinant prorenin (hrPR) for 15 min. Results are expressed as DCF probe fluorescence intensity versus total protein (mean ± SEM) (B). Protein levels of CTGF, TGF-β, and PAI-I represented by Western blot analysis (left) and quantitation (right) in M-1 cells incubated during 6 h with hrPR, GTK, or hrPR plus GTK. *p < 0.05 versus control, # p < 0.05 versus hrPR group, ‡ p < 0.05 versus positive control, n = 5.