Skip to main content
. 2019 Jul 23;10:803. doi: 10.3389/fphar.2019.00803

Figure 4.

Figure 4

Selective COX-2 inhibitor NS-398 prevented NOX-4 upregulation, the induction of ROS, and the increase in profibrotic markers CTGF, TGF-β, and PAI-I. (A). A dose response effect of COX-2 inhibitor NS-398 on NOX-4 protein expression in M-1 cells incubated with hrPR. (B). For ROS quantification, M-1 cells were treated with NS-398 (10−5 M) during 15 min, incubated with DCFH-DA probe for 30 min, and treated with hrPR for 15 min. Results are expressed as DCF probe fluorescence intensity versus total protein (mean ± SEM) (C). Protein levels of CTGF, TGF-β, and PAI-I represented by Western blot analysis (left) and quantitation (right) in M-1 cells incubated during 6 h with hrPR, NS-398, or hrPR plus NS-398, *p < 0.05 versus control, # p < 0.05 versus hrPR group, ‡p < 0.05 versus positive control, n = 5.