Figure 4.

The expression status of CD80⁺ and CD86⁺ on CD11c⁺ dendritic cells from spleens. Representative flow cytometry plots showing percentages of CD11c⁺CD80⁺ cells (A) and CD11c⁺CD86⁺ cells (B). From left to right are groups of Hepes, free DOX & IMQ, LT-DOX, LT-DOX+LT-IMQ, LT-DOX+LT-IMQ+anti-PD-L1. Proportions of CD11c⁺CD80⁺ cells (C) and CD11c⁺CD86⁺ cells (D). (E) The cytotoxicity assay of CD8⁺ T lymphocytes against 4T1 cells. Data are mean ± SD, n = 3; ^*P < 0.05; ^**P < 0.01; and ^***P < 0.001.