Figure 5.

Representative flow cytometry plots showing percentages of tumor-infiltrating CD3e⁺CD4⁺ killer T cells (A), CD3e⁺CD8⁺ effector T cells (B) and CD4⁺FoxP3⁺ regulatory T cells (C) in solid tumors after various treatments indicated. From left to right are groups of Hepes, free DOX & IMQ, LT-DOX, LT-DOX+LT-IMQ, LT-DOX+LT-IMQ+anti-PD-L1. Proportions of CD3e⁺CD4⁺ effector T cells (C), CD3e⁺CD8⁺ T cells (D) and CD4⁺FoxP3⁺ regulatory T cells (E). (G) CD4⁺CTLs/T_reg ratios and CD8⁺ effector T cells/T_reg cells ratios in the solid tumors upon various treatments. Cytokine levels of TNF-α (H), and IFN-γ (I) in sera from mice isolated on day 3 after various treatments. Three mice were measured in each group in A–I. Data are mean ± SD, n = 3; ^*P < 0.05; ^**P < 0.01; and ^***P < 0.001.