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. 2019 Jul 23;10:1709. doi: 10.3389/fimmu.2019.01709

Figure 5.

Figure 5

The binding capabilities of peptides with HLA-A*30:01, HLA-A*30:03, and the mutants HLA-A*30:01D77N, or HLA-A*30:03N77D. Gel filtration chromatograms (A) and Thermal stabilities tested by circular dichroism spectroscopy (B) of HLA-A*30:01/RT313 (red solid line), HLA-A*30:03/RT313 (black solid line), HLA-A*30:01D77N/RT313 (red dashed line), and HLA-A*30:03N77D/RT313 (black dashed line). (C,D) Gel filtration chromatograms (C) and Thermal stabilities (D) of HLA-A*30:01/MTB (blue solid line), HLA-A*30:03/MTB (green solid line), HLA-A*30:01D77N/MTB (blue dashed line), and HLA-A*30:03N77D/MTB (green dashed line). (E,F) Gel filtration chromatograms (E) and Thermal stabilities (F) of HLA-A*30:01/NP44 (magenta solid line), HLA-A*30:03/NP44 (orange solid line), HLA-A*30:01D77N/NP44 (magenta dashed line), and HLA-A*30:03N77D/NP44 (orange dashed line). The arrow shows the direction of the change in thermal stability after mutagenesis.