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. 2019 Jul 23;10:1707. doi: 10.3389/fimmu.2019.01707

Figure 3.

Figure 3

Localization of Fasciola gigantica-derived rFgCatB protein on the surface of PBMCs. Cells were treated with rFgCatB and incubated with rabbit anti-rFgCatB primary antibody. Hoechst (blue) and Cy3-conjugated secondary antibody (red) were used to stain host cell nuclei and rFgCatB protein, respectively. Surface localization was observed in rFgCatB-treated cells, whereas no staining was detected in untreated (control) cells. Scale bars, 10 μm.