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. 2019 Jul 23;10:1707. doi: 10.3389/fimmu.2019.01707

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Copyright © 2019 Chen, Tian, Hou, Li, Tian, Yuan, Li, Elsheikha and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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rFgCatB protein stimulated the production of cytokines. Goat PBMCs were incubated for 72 h in the presence of PBS or serial concentrations of rFgCatB protein. The levels of cytokines in the supernatant of cultured PBMCs were quantified by ELISA. Results showed that rFgCatB induced the expression of all examined cytokines in a dose-dependent manner. Graphs represent means ± standard deviations of data from three independent biological replicates (IL-2 (10 μg/ml: F_{(5, 36)} = 46.98, P < 0.0001; 20 μg/ml: F_{(5, 36)} = 46.98, P < 0.0001; 40 μg/ml: F_{(5, 36)} = 46.98, P < 0.0001; 80 μg/ml: F_{(5, 36)} = 46.98, P < 0.0001; 160 μg/ml: F_{(5, 36)} = 46.98, P < 0.0001), IL-4 (10 μg/ml: F_{(5, 36)} = 43.65, P < 0.0001; 20 μg/ml: F_{(5, 36)} = 43.65, P < 0.0001; 40 μg/ml: F_{(5, 36)} = 43.65, P < 0.0001; 80 μg/ml: F_{(5, 36)} = 43.65, P < 0.0001; 160 μg/ml: F_{(5, 36)} = 43.65, P < 0.0001), IL-10 (10 μg/ml: F_{(5, 36)} = 40.51, P = 0.0012; 20 μg/ml: F_{(5, 36)} = 40.51, P < 0.0001; 40 μg/ml: F_{(5, 36)} = 40.51, P < 0.0001; 80 μg/ml: F_{(5, 36)} = 40.51, P < 0.0001; 160 μg/ml: F_{(5, 36)} = 40.51, P < 0.0001), IL-17 (10 μg/ml: F_{(5, 36)} = 77.39, P = 0.0002; 20 μg/ml: F_{(5, 36)} = 77.39, P < 0.0001; 40 μg/ml: F_{(5, 36)} = 77.39, P < 0.0001; 80 μg/ml: F_{(5, 36)} = 77.39, P < 0.0001; 160 μg/ml: F_{(5, 36)} = 77.39, P < 0.0001), TGF-β (10 μg/ml: F_{(5, 36)} = 137.5, P < 0.0001; 20 μg/ml: F_{(5, 36)} = 137.5, P < 0.0001; 40 μg/ml: F_{(5, 36)} = 137.5, P < 0.0001; 80 μg/ml: F_{(5, 36)} = 137.5, P < 0.0001; 160 μg/ml: F_{(5, 36)} = 137.5, P < 0.0001), and IFN-γ (10 μg/ml: F_{(5, 36)} = 96.82, P = 0.0003; 20 μg/ml: F_{(5, 36)} = 96.82, P < 0.0001; 40 μg/ml: F_{(5, 36)} = 96.82, P < 0.0001; 80 μg/ml: F_{(5, 36)} = 96.82, P < 0.0001; 160 μg/ml: F_{(5, 36)} = 96.82, P < 0.0001). Asterisks indicate statistical significance between treated and untreated control PBMCs (^**P < 0.01; ^***P < 0.001; ^****P < 0.0001 compared with control).