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. 2019 Jul 23;10:1707. doi: 10.3389/fimmu.2019.01707

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Copyright © 2019 Chen, Tian, Hou, Li, Tian, Yuan, Li, Elsheikha and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of rFgCatB protein on the production of NO. PBMCs were treated with PBS or with serial concentrations of rFgCatB protein and maintained at 37°C for 24 h. NO concentration was measured by Griess assay. Graphs represent means ± standard deviations of data from three independent biological replicates (10 μg/ml: ANOVA, F_{(4, 10)} = 27.91, P = 0.9998; 20 μg/ml: ANOVA, F_{(4, 10)} = 27.91, P = 0.9702; 40 μg/ml: ANOVA, F_{(4, 10)} = 27.91, P = 0.0220; 80 μg/ml: ANOVA, F_{(4, 10)} = 27.91, P < 0.0001). Asterisks indicate significant differences between rFgCatB-treated and PBS-treated control cells (^*p < 0.05; ^****P < 0.0001; ns, non-significant compared with control).