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. 2019 Jul 23;10:1707. doi: 10.3389/fimmu.2019.01707

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Copyright © 2019 Chen, Tian, Hou, Li, Tian, Yuan, Li, Elsheikha and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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rFgCatB protein induced apoptosis in goat PBMCs. Annexin V/PI staining was used to quantify apoptotic cells by flow cytometry. (A) The FACS plot showing apoptosis of PBMCs in response to exposure to rFgCatB protein. (B) Apoptotic cells (Annexin V+/PI-) were plotted and compared with the percentage of cell population. Graphs represent means ± standard deviations of data from three independent biological replicates (10 μg/ml: ANOVA, F_{(4, 10)} = 34.92, P = 0.0006; 20 μg/ml: ANOVA, F_{(4, 10)} = 34.92, P = 0.0003; 40 μg/ml: ANOVA, F_{(4, 10)} = 34.92, P < 0.0001; 80 μg/ml: ANOVA, F_{(4, 10)} = 34.92, P < 0.0001). The asterisks indicate significant differences between rFgCatB-treated and PBS-treated control goat PBMCs (^***P < 0.001; ^****P < 0.0001 compared with control).