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. 2019 Jul 23;10:1726. doi: 10.3389/fimmu.2019.01726

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Copyright © 2019 Noack, Beringer and Miossec.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of cytokine interactions on endothelial cells. Endothelial cells (HUVEC) were cultured during 48 h in presence or not of different cytokine treatments: IL-17A (50 ng/ml), IL-17F (50 ng/ml), TNF (1 ng/ml) or IL-1β (10 pg/ml), cytokine alone or in combination, IL-17A+TNF, IL-17F+TNF, IL-17A+IL-1β, IL-17F+IL-1β, IL-17A+TNF+IL-1β or IL-17F+TNF+IL-1β. The production of IL-6 (A) and IL-8 (B) was measured by ELISA in the supernatants after 48 h. ^*p ≤ 0.05, ^*compares with control, ^#compares conditions. Results are represented as mean ± SEM. n = 3. Triplicates were done in each experiment. ^**,^##p ≤ 0.01.