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. 2019 Jul 23;10:1726. doi: 10.3389/fimmu.2019.01726

Figure 5.

Figure 5

Effect of cytokine interactions on hepatocytes. Hepatocytes were cultured during 48 h in presence or not of different cytokine treatments: IL-17A (50 ng/ml), IL-17F (50 ng/ml), TNF (1 ng/ml) or IL-1β (10 pg/ml), cytokine alone or in combination, IL-17A+TNF, IL-17F+TNF, IL-17A+IL-1β, IL-17F+IL-1β, IL-17A+TNF+IL-1β or IL-17F+TNF+IL-1β. The production of IL-6 (A) and IL-8 (B) was measured by ELISA in the supernatants after 48 h. *p ≤ 0.05, *compares with control, #compares conditions. Results are represented as mean ± SEM. n = 3. Duplicates were done in each experiment.