Figure 1.

Functional TRESK channels are present in all TG and DRG neurons. A, Schematics of WT mouse TRESK/Kcnk18 allele and the targeted allele. After homologous recombination, the lacZ-loxP-hubiP-neo-loxP cassette replaces most of the TRESK coding region. B, Representative images of TG and DRG sections from adult WT and TRESK KO mice double stained with antibodies against TRESK and βIII tubulin. βIII tubulin-IR is present in all neurons. TRESK-IR is present in almost all neurons in the WT sections. There is no TRESK-IR in the KO sections. C, Total number of TG neurons in WT and TRESK KO mice (n = 3 mice in each group). D, The percentage of IB4⁺ and IB4^– neurons in WT and KO TG culture (1010 WT and 940 KO TG neurons from 10 separate primary cultures were counted).