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. 2019 Jul 26;6(4):ENEURO.0236-19.2019. doi: 10.1523/ENEURO.0236-19.2019

Figure 8.

Figure 8.

The small IB4 dural afferent neurons from TRESK KO mice show higher intrinsic excitability. A, Representative traces of APs generated by incremental depolarizing current injections in FG+ dural afferent neurons from WT and TRESK KO mice. The values of Vrest and the current amplitude are indicated. B, C, Mean rheobase (B) and Rin (C) of FG+ small IB4 and IB4+ dural afferent neurons from WT and KO mice (n = 13–20 neurons in each group; for details of the intrinsic properties of dural afferent neurons, see Table 2). ***p < 0.001; two-tailed t test between the corresponding WT and KO groups. D, E, Input/output plots of the spike frequency in response to incremental depolarizing current injections in small IB4 (D) and small IB4+ (E) dural afferent neurons from WT and KO mice (same neurons as in B). ##p < 0.01; two-way RM ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001; two-tailed t test between the corresponding WT and KO groups. F, The density of Iba1+ macrophages in WT and KO TG (n = 3 mice in each group). G, The mean area of individual Iba1+ macrophages in WT and KO TG (same mice as in F). H, The area of Iba1+ macrophages per mm2 TG in WT and KO mice (same mice as in F). I, Cumulative distributions of the cross-sectional areas of FG+ dural afferent neurons in WT and KO mice (n = 3627 and 3332 FG+ neurons pooled from 4 WT and 4 KO mice, respectively).