Table 1.
Intrinsic properties of TG neurons from adult WT, HET, and TRESK KO mice
| Diameter, μm | Capacitance, pF | Rin, MΩ | Vrest, mV | Rheobase, pA | AP threshold, mV | AP amplitude, mV | AP half-width, ms | AHP amplitude, mV | Cell number | |
|---|---|---|---|---|---|---|---|---|---|---|
| Small IB4-negative neurons | ||||||||||
| WT | 18.3 ± 0.6 | 18.0 ± 1.6 | 1249 ± 126 | −50.8 ± 1.1 | 47 ± 10 | −14.7 ± 1.9 | 104.5 ± 3.0 | 5.3 ± 0.6 | 19.6 ± 0.9 | 28 |
| HET | 18.6 ± 0.5 | 16.8 ± 1.8 | 1172 ± 74 | −52.8 ± 1.1 | 45 ± 9 | −16.4 ± 1.4 | 105.6 ± 1.5 | 3.5 ± 0.4 | 17.3 ± 1.0 | 16 |
| KOa | 18.1 ± 0.6 | 17.3 ± 1.7 | 1849 ± 262* | −50.1 ± 1.3 | 18 ± 4** | −18.5 ± 1.7 | 98.9 ± 2.7 | 4.7 ± 0.5 | 17.5 ± 1.8 | 17 |
| Small IB4-positive neurons | ||||||||||
| WT | 19.2 ± 0.5 | 20.0 ± 1.2 | 997 ± 99 | −53.3 ± 1.6 | 103 ± 12 | −10.7 ± 2.6 | 110.2 ± 4.1 | 5.4 ± 0.8 | 19.8 ± 0.9 | 14 |
| HET | 22.5 ± 0.7 | 20.6 ± 1.6 | 1080 ± 149 | −54.7 ± 2.0 | 99 ± 11 | 16.1 ± 1.9 | 112.2 ± 2.8 | 5.7 ± 0.7 | 17.2 ± 1.0 | 17 |
| KO | 20.1 ± 1.0 | 22.8 ± 2.9 | 1507 ± 205* | −53.4 ± 1.4 | 70 ± 10* | 13.6 ± 1.4 | 107.8 ± 3.8 | 6.9 ± 0.7 | 18.2 ± 2.2 | 14 |
| Medium-sized neurons | ||||||||||
| WT | 30.8 ± 0.2 | 40.6 ± 2.5 | 593 ± 110 | −57.8 ± 0.9 | 243 ± 46 | 15.0 ± 2.5 | 119.5 ± 3.0 | 4.9 ± 0.5 | 16.6 ± 0.2 | 16 |
| KO | 29.3 ± 0.1 | 40.2 ± 2.7 | 754 ± 108 | −56.3 ± 1.1 | 207 ± 30 | 14.7 ± 1.8 | 113.5 ± 3.5 | 5.0 ± 0.7 | 17.6 ± 0.3 | 21 |
*p < 0.05, **p < 0.01; one-way ANOVA with post hoc Bonferroni test, compared with the corresponding WT and HET groups.
a
Neurons that exhibit spontaneous APs at Vrest are not included.