Figure 2.

Effect of Exogenous Cyanide on Individual Cultures

The P. aeruginosa wild-type (WT), ΔlasR, ΔlasR rhlR, and cyanide-sensitive ΔcioAB PA4129-34 strains were grown in individual LB cultures under low aeration, with and without exogenous cyanide as indicated. Cyanide was added at 100 μM final concentration upon inoculation, and growth was measured as optical density at 600 nm (OD₆₀₀) over time. Significant differences between strain densities at each time point were assessed by two-way ANOVA with post-hoc test, allowing the distinction of three groups as indicated: (1) all four strains without exogenous cyanide; (2) WT, ΔlasR, and ΔlasR rhlR with exogenous cyanide; and (3) ΔcioAB PA4129-34 with exogenous cyanide by itself. Cell densities differed significantly between strains from different groups, but not between strains within the same group (see Tables S1 and S5 for individual p values). Data points are the means of three independent biological replicates, with error bars indicating standard deviation. In one case, the bottom arm of an error bar is missing, because it has a negative value, and a negative value cannot be plotted on a log scale.