Figure 3.

Expression of Cyanide Resistance and Production Genes

β-Galactosidase activities of PA4130’-‘lacZ, PA4133’-‘lacZ, cioA’-‘lacZ, and hcnA’-‘lacZ reporter fusions were measured in the P. aeruginosa wild-type (WT), the ΔhcnB mutant, and the ΔlasR mutant, using a luminescence assay. Cultures were grown in LB medium under low aeration until early stationary phase (OD₆₀₀ of 1.0–1.5), with or without 25 μM potassium cyanide (blue and red bars, respectively). Relative β-galactosidase activities represent luminescence values normalized to OD₆₀₀. Data points are the means of three independent biological replicates, with error bars indicating standard deviation. See Tables S1 and S6 for complete statistics and pairwise comparisons.