FIGURE 2.

PBMCs migration induced by inflammatory cytokines is prevented by a short-term exposure to Aβ. Endothelial cells co-cultured with astrocytes were exposed to Aβ 1–42 (Aβ, 2.5 μM), TNFα+IFNγ (10 U/ml and 5 U/ml respectively, T&I) or their combination (Aβ+T&I) for 5 (A) or 18 h (B). Effects of treatments were examined by evaluating the number of PBMCs migrating through the barrier after 18 h. PBMCs migration represents the ratio of migrated cells through the barrier, given a constant input, expressed as percentage of migration in control conditions. Data are mean ± SEM of 5 (A) or 3 (B) independent experiments, each run in duplicate. ^*p < 0.05 versus control, ^$p < 0.05 versus T&I. Significance was assessed by one-way ANOVA followed by Newman–Keuls test.