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. 2019 Jul 23;13:337. doi: 10.3389/fncel.2019.00337

FIGURE 3.

FIGURE 3

Aβ treatment affects the expression of VE-cadherin and prevents T&I-induced overexpression of hypermannosylated ICAM-1. Endothelial astrocytes co-cultures were exposed for 5 h to Aβ (2.5 μM), T&I (10 U/ml and 5 U/ml respectively) or their combination (Aβ+T&I), and the expression and cellular localization of VE-cadherin were examined by western blot (A) and immunofluorescence analysis (B). ICAM-1 mRNA levels were evaluated in endothelial cells co-cultured with astrocytes and exposed to treatments for 5 h (C). Protein expression of ICAM-1 in endothelial cells co-cultured with astrocytes and exposed to treatments was evaluated by western blot analysis. Densitometric analysis of the two isoform bands (85 and 75 kDa) are reported in (D). mRNA levels are reported as fold increase versus control (C). Ve-cadherin is represented in red, while 4′6-diamidino-2-phenylindole (DAPI) is used to counterstain nuclei. Scale bar = 10 μm. Data are mean ± SEM of 4 (A) 3 (C) or 6 (D) independent experiments. *p < 0.05 versus control, $p < 0.05 versus T&I. Significance was assessed by one-way ANOVA followed by Newman–Keuls test.