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. 2019 Jul 23;13:337. doi: 10.3389/fncel.2019.00337

FIGURE 4.

FIGURE 4

PBMCs migration through endothelial monolayer is induced by Aβ treatments under inflammatory conditions. PBMCs migration through a barrier constituted by endothelial mono-cultures exposed for 5 h to Aβ (2.5 μM), T&I (10 U/ml and 5 U/ml respectively) or their combination (Aβ+T&I), is evaluated after 18 h migration assay (A). Western blot expression (B) and immunocytochemical analysis of VE-cadherin (C), as well as and ICAM-1 mRNA levels (D) and protein expression (E) were evaluated in endothelial mono-cultures exposed to treatments for 5 h. PBMCs migration represents the ratio of migrated cells through the barrier given a constant input after 18 h assay, and then expressed as percentage of migration in control conditions (A). Data are mean ± SEM of 3 (A, each run in duplicate, and C) or 4 (B,D) independent experiments. *p < 0.05 versus control. Significance was assessed by one-way ANOVA followed by Newman–Keuls test.