FIGURE 5.

Endothelial expression of Gnt-III is differently modulated in the presence of astrocytes. The expression of ICAM-1 after 5 U PNGase enzymatic digestion was evaluated by electrophoretic separation of lysates derived from 5 h T&I-exposed endothelial co-cultured cells (A). mRNA levels of Gnt-III were evaluated in endothelial cells both co-cultured with astrocytes (B) or in mono-cultures (C) and exposed for 5 h to Aβ (2.5 μM), T&I (10 and 5 U/ml respectively) or their combination (Aβ+T&I). The expression of miR-200b was examined in astrocytes co-cultured with endothelial cells (D), endothelial cells co-cultured with astrocytes (E), or endothelial mono-cultures (F), all exposed to treatments for 5 h. Values are expressed as fold increase versus ctr. Data are mean ± SEM of 3 independent experiments. ^*p < 0.05 versus control, ^$p < 0.05 versus T&I. Significance was assessed by one-way ANOVA followed by Newman–Keuls test.