Figure 1.

MK-801 suppresses brain-derived neurotrophic factor (BDNF) mRNA expression and protein secretion in primary cultured hippocampal neurons. (A,B) Hippocampal neurons were cultured in the presence or absence of 20 μM MK-801 for 24 h and BDNF mRNA and protein levels in cell lysates and culture supernatant were measured by quantitative real-time (qRT-PCR; A) and enzyme-linked immunosorbent assay (ELISA; B), respectively. Densitometric values are expressed as mean ± SEM of three independent experiments. **P < 0.01 vs. control group (Student’s t-test).