Figure 3.

A guanine base at position −1 of the PFS helps to prevent DNA cleavage. A, scatter plots showing the fraction of DNA cleaved by the TmaCmr complex loaded with WT and crRNAs with mutations at position −1 (C-1U, C-1A, and C-1G). RNA targets contained U, C, A, or G at position −1. All targets have the sequence UUAGGUA in positions −2 to −8. B, scatter plots of the fraction DNA cleaved by TmaCmr complex when activated by transcripts containing PFSs with varying amounts of anti-tag sequence. A and B, black letters indicate positions of anti-tag sequence. Individual values are plotted as solid black circles. Bar chart represents the mean of these values. Independent experiments were repeated at least three times. Error bars are S.D.