Figure 1.

Bypass across and extension past dG, 2′-F dG, and N⁷-CH₃ 2′-F dG. A, hpol η; B, hpol κ; C, hpol ι; D, Dpo4. The sequences of the template and primer are shown at the top. Reactions contained 200 nm FAM-labeled primer–template oligonucleotide complex, 250 μm dNTPs, and 20 nm enzyme, except for pol ι (40 nm). Reactions were done at 37 °C for 2, 5, 10, 20, and 60 min.