Figure 2.

Single-nucleotide incorporation opposite dG, 2′-F dG, and N⁷-CH₃ 2′-F dG. A, hpol η; B, hpol κ; C, hpol ι; D, Dpo4. The sequences of the template and primer are shown at the top. Reactions were conducted with 120 nm FAM-labeled primer–template oligonucleotide complex, 250 μm dNTPs, and 5 nm enzyme except for pol ι (10 nm). Reactions were conducted at 37 °C for 10 min.