Figure 5.

Steady-state kinetic analysis of dATP extension by hpol η. A, 2′-F dG:dC; B, N⁷-CH₃ 2′-F dG:dC; C, 2′-F dG:dT; D, N⁷-CH₃ 2′-F dG:dT bp. The sequences of the template and primer are shown at the top. Reactions were done at 37 °C for 5–10 min by incubating 120 nm primer–template oligonucleotide complex, 5–10 nm hpol η, and varying concentrations of dNTPs.