Table 4.
LC-ESI-MS/MS analysis of full-length extension products across N7-CH3 2′-F dG by hpol η
The oligonucleotides used were as follows,
5′-FAM-CGGGCTCGTAAGCGTCUT-3′ 3′-GCCCGAGCATTCGCAGTAXTACT-5′
where X represents 2′-F dG and N7-CH3 2′-F dG. Products were cut at U, and the expected sequences began at the 3′ T of the primer.
| Product sequence | m/z, observed (charge) | m/z, theoretical (charge) | Relative peak area/tR (min) | % |
|---|---|---|---|---|
| 5′-pTCATGA | 934.27 (−2) | 1,870.22 (−1), 934.60 (−2) | 5,585.4/1.68 | 85 |
| 5′-pTCATGAT | 1,086.26 (−2), 724.07 (−3) | 1,086.70 (−2), 724.13 (−3) | 1,838.2/1.91 | |
| 5′-pTCAT | 613.22 (−2) | 1,227.80 (−1), 613.39 (−2) | 13,511/1.73 | |
| 5′-pTACTGA | 934.27 (−2) | 1,870.22 (−1), 934.60 (−2) | 620.6/1.68 | 10 |
| 5′-pTAGTCAT | 1,086.26 (−1), 724.07 (−2) | 1,086.70 (−1), 724.13 (−2) | 2,095.5/1.91 | |
| 5′-pTGATCAT | 1,086.26 (−1), 724.07 (−2) | 1,086.70 (−1), 724.13 (−2) | 1,397/1.91 | 5 |