Table 5.
LC-ESI-MS/MS analysis of full-length extension products across N7-CH3 2′-F dG by Dpo4
The oligonucleotides used were as follows,
5′-FAM-CGGGCTCGTAAGCGTCUT-3′ 3′-GCCCGAGCATTCGCAGTAXTACT-5′
where X represents 2′-F dG and N7-CH3 2′-F dG. Products were cut at U, and expected sequences began at 3′ T.
| Product sequence | m/z, observed (charge) | m/z, theoretical (charge) | Relative peak area/tR (min) | % |
|---|---|---|---|---|
| 5′-pTCATGA | 934.22 (−2) | 1,870.22 (−1), 934.60 (−2) | 6,417/ 1.67 | 100 |
| 5′-pTCATG | 777.56 (−2) | 1,557.01 (−1), 777.99 (−2) | 10,707/1.57 | |
| 5′-pTCAT | 613.09 (−2) | 1,227.80 (−1), 613.39 (−2) | 12,253/1.73 |