Figure 6.

Stereoselectivity of the mouse liver microsomal sn-1 acyltransferase reaction to produce 1-stearoyl-2-AA-ether-PC. Microsomal homogenates isolated from mouse liver were incubated with either 10 μm l-2-AA-ether-LPC or 10 μm d-2-AA-ether-LPC in the presence of 10 μm 18:0-CoA for 0, 2, 5, 9, or 15 min at 37 °C in 75 mm sodium phosphate buffer (pH 7.4). The reactions were terminated by adding chloroform/methanol (1:1, v/v) and vortexed. The chloroform phase was isolated and dried under a nitrogen stream. The dried residues were redissolved in water/methanol (1:4), and the sn-1 acyltransferase product, 1-stearoyl-2-AA-ether-PC, was analyzed and quantitated by LC-MS in the positive ion mode. Values are the average of four independent preparations. Error bars represent S.D.