Table 3.
Parameters derived from SVD–EFA SEC–SAXS analysis of C29S
Sample | Guinier |
GNOM |
Pxb | Mass (kDa) |
||||
---|---|---|---|---|---|---|---|---|
qRg | Rga | Rg | Dmax | T.E. | By Qrc | By Porodd | ||
Å | Å | Å | ||||||
No Phe | 0.35 − 1.26 | 41.4 ± 0.6 | 41.0 | 135 | 0.85 | 3.9 | 191 | 207 |
1 mm Phe | 0.48 − 1.26 | 40.5 ± 5.2 | 41.2 | 131 | 0.93 | 4.0 | 218 | 234 |
a Errors reported reflect the uncertainty in the value for Rg determined using classical Guinier fitting.
b Px is Porod exponent. Values near ∼4 indicate compactness, whereas lower values between 2 and 3 indicate significant lack of compactness and increased volumes (28). These values were determined using the program ScÅtter (https://bl1231.als.lbl.gov/scatter/).
c Mass determinations using the Qr invariant (26) were determined using the program RAW.
d Mass determinations using the Porod volume (27) were determined using the program RAW.