Table 1.
Primers used for PON2 and PON3 polymorphic positions C311S, G148A and C133A respectively.
| Polymorphic sites | Primer Sequence | PCR Products | Restriction Enzymes | Restricted bands | |
|---|---|---|---|---|---|
| PON 2 | C311S | 5′- ACATGCATGTACGGTGGTCT-3′ (forward) 5′-AGCAATTCATAGATTAATTGTTA-3′ (reverse) |
142 bp 120 bp* |
DdeI | C allele: 142 bp S allele: 75 bp + 67 bp |
| G148A | 5′-AGTGGAAATTTTTAAATTTGAAGCAG-3′ (forward) 5′-TTGTTTGCAAATGCTGGGGAT-3′ (reverse) |
130 bp | Fnu4HI | G allele: 130 bp A allele: 110 bp + 20 bp |
|
| PON 3 | C133A | 5′-TTACCTATCATGTAGACTGTGAGA*GT-3′ (forward) 5′-ATTCACAACATAAAGATACACAGTA-3′ (reverse) |
168 bp | HinfI | A allele: 168 bp C allele: 143 bp |
*
120 bp band found in all genotype because of common restriction site.