Table 3: Cytokines detected that display moderate to profound variability.

Thirteen (13) of the 36 cytokines detected in eosinophil lysates that display moderate variability (n = 14 donors). Profiling included standard donor lysate (see Fig. 1) as a normalization control as described in the Legend to Table 2. Moderate variability is defined as coefficient of variation (CV) > 50% and ≤ 100%; see Fig. 2 for an example of how this analysis was performed; npd, normalized pixel density. One cytokine (IL-16) of the 36 detected displays profound variability, with a CV > 100%.

Cytokine	CV (%)	Mean ± SD (npd)	Range (npd)
IFN-γ	50.8	0.85 ± 0.41	0.23 – 1.63
IL-6	53.5	1.97 ± 1.05	0.44 – 3.39
IL-18	54.9	1.37 ± 0.75	0.39 – 2.56
IL-8	55.8	1.64 ± 0.91	0.56 – 3.85
IL-2	56.1	1.53 ± 0.86	0.47 – 2.82
IL-10	60.3	1.23 ± 0.74	0.24 – 2.62
IL-12p70	62.1	1.70 ± 1.05	0.47 – 3.76
IL-1β	63.1	1.77 ± 1.12	0.40 – 3.79
IL-1α	65.7	1.31 ± 0.86	0.33 – 3.11
TREM-1	66.7	1.29 ± 0.86	0.26 – 3.28
IL-27	72.5	1.80 ± 1.30	0.30 – 4.11
CXCL11	73.6	1.55 ± 1.14	0.31 – 3.94
IL-1Ra	84.1	1.22 ± 1.03	0.33 – 3.10
Cytokine	CV (%)	Mean ± SD (npd)	Range (npd)
IL-16	103	6.08 ± 6.3	0.49 – 19.1